Constructing next-generation CRISPR–Cas tools from structural blueprints

Bravo JPK, Hibshman GN, Taylor DW. 2022. Constructing next-generation CRISPR–Cas tools from structural blueprints. Current Opinion in Biotechnology. 78, 102839.

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Journal Article | Published | English

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Author
Bravo, JackISTA ; Hibshman, Grace N; Taylor, David W
Abstract
Clustered regularly interspaced short palindromic repeats - CRISPR-associated protein (CRISPR-Cas) systems are a critical component of the bacterial adaptive immune response. Since the discovery that they can be reengineered as programmable RNA-guided nucleases, there has been significant interest in using these systems to perform diverse and precise genetic manipulations. Here, we outline recent advances in the mechanistic understanding of CRISPR-Cas9, how these findings have been leveraged in the rational redesign of Cas9 variants with altered activities, and how these novel tools can be exploited for biotechnology and therapeutics. We also discuss the potential of the ubiquitous, yet often-overlooked, multisubunit CRISPR effector complexes for large-scale genomic deletions. Furthermore, we highlight how future structural studies will bolster these technologies.
Publishing Year
Date Published
2022-12-01
Journal Title
Current Opinion in Biotechnology
Volume
78
Article Number
102839
ISSN
IST-REx-ID

Cite this

Bravo JPK, Hibshman GN, Taylor DW. Constructing next-generation CRISPR–Cas tools from structural blueprints. Current Opinion in Biotechnology. 2022;78. doi:10.1016/j.copbio.2022.102839
Bravo, J. P. K., Hibshman, G. N., & Taylor, D. W. (2022). Constructing next-generation CRISPR–Cas tools from structural blueprints. Current Opinion in Biotechnology. Elsevier. https://doi.org/10.1016/j.copbio.2022.102839
Bravo, Jack Peter Kelly, Grace N Hibshman, and David W Taylor. “Constructing Next-Generation CRISPR–Cas Tools from Structural Blueprints.” Current Opinion in Biotechnology. Elsevier, 2022. https://doi.org/10.1016/j.copbio.2022.102839.
J. P. K. Bravo, G. N. Hibshman, and D. W. Taylor, “Constructing next-generation CRISPR–Cas tools from structural blueprints,” Current Opinion in Biotechnology, vol. 78. Elsevier, 2022.
Bravo JPK, Hibshman GN, Taylor DW. 2022. Constructing next-generation CRISPR–Cas tools from structural blueprints. Current Opinion in Biotechnology. 78, 102839.
Bravo, Jack Peter Kelly, et al. “Constructing Next-Generation CRISPR–Cas Tools from Structural Blueprints.” Current Opinion in Biotechnology, vol. 78, 102839, Elsevier, 2022, doi:10.1016/j.copbio.2022.102839.

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