Structural basis for assembly of non-canonical small subunits into type I-C Cascade

O’Brien RE, Santos IC, Wrapp D, Bravo JPK, Schwartz EA, Brodbelt JS, Taylor DW. 2020. Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. 11, 5931.

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Author
O’Brien, Roisin E.; Santos, Inês C.; Wrapp, Daniel; Bravo, JackISTA ; Schwartz, Evan A.; Brodbelt, Jennifer S.; Taylor, David W.
Abstract
Bacteria and archaea employ CRISPR (clustered, regularly, interspaced, short palindromic repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and degrade foreign nucleic acids. While a myriad of CRISPR-Cas systems have been identified to date, type I-C is one of the most commonly found subtypes in nature. Interestingly, the type I-C system employs a minimal Cascade effector complex, which encodes only three unique subunits in its operon. Here, we present a 3.1 Å resolution cryo-EM structure of the <jats:italic>Desulfovibrio vulgaris</jats:italic> type I-C Cascade, revealing the molecular mechanisms that underlie RNA-directed complex assembly. We demonstrate how this minimal Cascade utilizes previously overlooked, non-canonical small subunits to stabilize R-loop formation. Furthermore, we describe putative PAM and Cas3 binding sites. These findings provide the structural basis for harnessing the type I-C Cascade as a genome-engineering tool.
Publishing Year
Date Published
2020-11-23
Journal Title
Nature Communications
Volume
11
Article Number
5931
ISSN
IST-REx-ID

Cite this

O’Brien RE, Santos IC, Wrapp D, et al. Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. 2020;11. doi:10.1038/s41467-020-19785-8
O’Brien, R. E., Santos, I. C., Wrapp, D., Bravo, J. P. K., Schwartz, E. A., Brodbelt, J. S., & Taylor, D. W. (2020). Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-19785-8
O’Brien, Roisin E., Inês C. Santos, Daniel Wrapp, Jack Peter Kelly Bravo, Evan A. Schwartz, Jennifer S. Brodbelt, and David W. Taylor. “Structural Basis for Assembly of Non-Canonical Small Subunits into Type I-C Cascade.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-19785-8.
R. E. O’Brien et al., “Structural basis for assembly of non-canonical small subunits into type I-C Cascade,” Nature Communications, vol. 11. Springer Nature, 2020.
O’Brien RE, Santos IC, Wrapp D, Bravo JPK, Schwartz EA, Brodbelt JS, Taylor DW. 2020. Structural basis for assembly of non-canonical small subunits into type I-C Cascade. Nature Communications. 11, 5931.
O’Brien, Roisin E., et al. “Structural Basis for Assembly of Non-Canonical Small Subunits into Type I-C Cascade.” Nature Communications, vol. 11, 5931, Springer Nature, 2020, doi:10.1038/s41467-020-19785-8.
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