Crystallographic and spectroscopic studies of d(CCGGTACCGG)

Venkadesh S, Mandal PK, Gautham N. 2012. Crystallographic and spectroscopic studies of d(CCGGTACCGG). Nucleosides, Nucleotides and Nucleic Acids. 31(3), 184–196.

Download
No fulltext has been uploaded. References only!

DOI
10.1080/15257770.2011.648363
Journal Article | Published | English
Author
Venkadesh, S.; Mandal, Pradeep KISTA ; Gautham, N.
Abstract
The decanucleotide sequence d(CCGGTACCGG) crystallizes as a four-way junction at low cobalt ion concentrations (i.e., 1 mM). When the cobalt concentration in the crystallization solution is increased to 5 mM, the sequence crystallizes as resolved B-DNA duplexes. Gel retardation studies of the decamer show both a faint slow-moving band and a much thicker fast-moving band at low cobalt ion concentrations, and only the intense fast-moving band at higher ion concentration. Circular dichroism (CD) spectroscopy of the decamer indicates a structural transition as the cobalt ion concentration in the solution is increased, probably from B-type to A-type DNA. These studies revealed that the oligomer sequence has several conformations and structures accessible to it, in a manner dependent on sequence, ion concentration, and DNA concentration.
Publishing Year
2012
Date Published
2012-03-01
Journal Title
Nucleosides, Nucleotides and Nucleic Acids
Publisher
Informa UK Limited
Volume
31
Issue
3
Page
184-196
ISSN
1525-7770
eISSN
1532-2335
IST-REx-ID
21151

Cite this

Venkadesh S, Mandal PK, Gautham N. Crystallographic and spectroscopic studies of d(CCGGTACCGG). Nucleosides, Nucleotides and Nucleic Acids. 2012;31(3):184-196. doi:10.1080/15257770.2011.648363
Venkadesh, S., Mandal, P. K., & Gautham, N. (2012). Crystallographic and spectroscopic studies of d(CCGGTACCGG). Nucleosides, Nucleotides and Nucleic Acids. Informa UK Limited. https://doi.org/10.1080/15257770.2011.648363
Venkadesh, S., Pradeep K Mandal, and N. Gautham. “Crystallographic and Spectroscopic Studies of d(CCGGTACCGG).” Nucleosides, Nucleotides and Nucleic Acids. Informa UK Limited, 2012. https://doi.org/10.1080/15257770.2011.648363.
S. Venkadesh, P. K. Mandal, and N. Gautham, “Crystallographic and spectroscopic studies of d(CCGGTACCGG),” Nucleosides, Nucleotides and Nucleic Acids, vol. 31, no. 3. Informa UK Limited, pp. 184–196, 2012.
Venkadesh S, Mandal PK, Gautham N. 2012. Crystallographic and spectroscopic studies of d(CCGGTACCGG). Nucleosides, Nucleotides and Nucleic Acids. 31(3), 184–196.
Venkadesh, S., et al. “Crystallographic and Spectroscopic Studies of d(CCGGTACCGG).” Nucleosides, Nucleotides and Nucleic Acids, vol. 31, no. 3, Informa UK Limited, 2012, pp. 184–96, doi:10.1080/15257770.2011.648363.

Export

Marked Publications

Open Data ISTA Research Explorer

Sources

PMID: 22356234
PubMed | Europe PMC

Search this title in

Google Scholar