Florian M Praetorius
Praetorius Group
18 Publications
2024 | Published | Journal Article | IST-REx-ID: 17463 |

A. Pillai et al., “De novo design of allosterically switchable protein assemblies,” Nature, vol. 632. Springer Nature, pp. 911–920, 2024.
[Published Version]
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| Files available
| DOI
2023 | Published | Journal Article | IST-REx-ID: 14281
Praetorius FM, Leung PJY, Tessmer MH, Broerman A, Demakis C, Dishman AF, Pillai A, Idris A, Juergens D, Dauparas J, Li X, Levine PM, Lamb M, Ballard RK, Gerben SR, Nguyen H, Kang A, Sankaran B, Bera AK, Volkman BF, Nivala J, Stoll S, Baker D. 2023. Design of stimulus-responsive two-state hinge proteins. Science. 381(6659), 754–760.
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| DOI
| PubMed | Europe PMC
2023 | Submitted | Preprint | IST-REx-ID: 14294 |

Edman NI, Redler RL, Phal A, Schlichthaerle T, Srivatsan SR, Etemadi A, An S, Favor A, Ehnes D, Li Z, Praetorius FM, Gordon M, Yang W, Coventry B, Hicks DR, Cao L, Bethel N, Heine P, Murray AN, Gerben S, Carter L, Miranda M, Negahdari B, Lee S, Trapnell C, Stewart L, Ekiert DC, Schlessinger J, Shendure J, Bhabha G, Ruohola-Baker H, Baker D. Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies. bioRxiv, 10.1101/2023.03.14.532666.
[Preprint]
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| DOI
| Download Preprint (ext.)
2022 | Published | Journal Article | IST-REx-ID: 14282
D. D. Sahtoe et al., “Reconfigurable asymmetric protein assemblies through implicit negative design,” Science, vol. 375, no. 6578. American Association for the Advancement of Science, 2022.
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| DOI
| PubMed | Europe PMC
2019 | Published | Journal Article | IST-REx-ID: 14299 |

E. FAS et al., “Custom-size, functional, and durable DNA origami with design-specific scaffolds,” ACS Nano, vol. 13, no. 5. ACS Publications, pp. 5015–5027, 2019.
[Published Version]
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| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2018 | Published | Journal Article | IST-REx-ID: 14284 |

B. Bräuning et al., “Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB,” Nature Communications, vol. 9. Springer Nature, 2018.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2018 | Published | Thesis | IST-REx-ID: 14306 |

F. M. Praetorius, “Genetically encoding the spatial arrangement of DNA and proteins in self-assembling nanostructures,” Technische Universität München, 2018.
[Published Version]
View
| Download Published Version (ext.)
2017 | Published | Journal Article | IST-REx-ID: 14286
B. Kick, S. Hensler, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Specific growth rate and multiplicity of infection affect high-cell-density fermentation with bacteriophage M13 for ssDNA production,” Biotechnology and Bioengineering, vol. 114, no. 4. Wiley, pp. 777–784, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14287
F. M. Praetorius and H. Dietz, “Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes,” Science, vol. 355, no. 6331. American Association for the Advancement of Science, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14290
F. M. Praetorius, B. Kick, K. L. Behler, M. N. Honemann, D. Weuster-Botz, and H. Dietz, “Biotechnological mass production of DNA origami,” Nature, vol. 552, no. 7683. Springer Nature, pp. 84–87, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14309 |

M. Siavashpouri, C. Wachauf, M. Zakhary, F. M. Praetorius, H. Dietz, and Z. Dogic, “Molecular engineering of chiral colloidal liquid crystals using DNA origami,” Nature Materials, vol. 16, no. 8. Springer Nature, pp. 849–856, 2017.
[Preprint]
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| DOI
| Download Preprint (ext.)
| PubMed | Europe PMC
| arXiv
2017 | Published | Conference Abstract | IST-REx-ID: 14310
M. Siavashpouri, C. Wachauf, M. Zakhary, F. M. Praetorius, H. Dietz, and Z. Dogic, “Molecular engineering of colloidal liquid crystals using DNA origami,” in APS March Meeting 2017, 2017.
View
2016 | Published | Journal Article | IST-REx-ID: 14302
E. Stahl, F. M. Praetorius, C. C. de Oliveira Mann, K.-P. Hopfner, and H. Dietz, “Impact of heterogeneity and lattice bond strength on DNA triangle crystal growth,” ACS Nano, vol. 10, no. 10. American Chemical Society, pp. 9156–9164, 2016.
View
| DOI
| PubMed | Europe PMC
2016 | Published | Journal Article | IST-REx-ID: 14304
T. G. Martin et al., “Design of a molecular support for cryo-EM structure determination,” PNAS, vol. 113, no. 47. Proceedings of the National Academy of Sciences, pp. E7456–E7463, 2016.
[Published Version]
View
| DOI
| PubMed | Europe PMC
2015 | Published | Journal Article | IST-REx-ID: 14303 |

B. Kick, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Efficient production of single-stranded phage DNA as scaffolds for DNA origami,” Nano Letters, vol. 15, no. 7. ACS Publications, pp. 4672–4676, 2015.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2014 | Published | Journal Article | IST-REx-ID: 14301 |

E. Stahl, T. Martin, F. M. Praetorius, and H. Dietz, “Facile and scalable preparation of pure and dense DNA origami solutions,” Angewandte Chemie International Edition, vol. 126, no. 47. Wiley, pp. 12949–12954, 2014.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2011 | Published | Journal Article | IST-REx-ID: 14305 |

A. Bachmann, D. Wildemann, F. M. Praetorius, G. Fischer, and T. Kiefhaber, “Mapping backbone and side-chain interactions in the transition state of a coupled protein folding and binding reaction,” PNAS, vol. 108, no. 10. Proceedings of the National Academy of Sciences, pp. 3952–3957, 2011.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
1 Grants
Design of Nucleic Acid-Templated Ordered Protein Assemblies (DNA-TO Pass)
2024-03-01 – 2029-28-02
HE_ERC
18 Publications
2024 | Published | Journal Article | IST-REx-ID: 17463 |

A. Pillai et al., “De novo design of allosterically switchable protein assemblies,” Nature, vol. 632. Springer Nature, pp. 911–920, 2024.
[Published Version]
View
| Files available
| DOI
2023 | Published | Journal Article | IST-REx-ID: 14281
Praetorius FM, Leung PJY, Tessmer MH, Broerman A, Demakis C, Dishman AF, Pillai A, Idris A, Juergens D, Dauparas J, Li X, Levine PM, Lamb M, Ballard RK, Gerben SR, Nguyen H, Kang A, Sankaran B, Bera AK, Volkman BF, Nivala J, Stoll S, Baker D. 2023. Design of stimulus-responsive two-state hinge proteins. Science. 381(6659), 754–760.
View
| DOI
| PubMed | Europe PMC
2023 | Submitted | Preprint | IST-REx-ID: 14294 |

Edman NI, Redler RL, Phal A, Schlichthaerle T, Srivatsan SR, Etemadi A, An S, Favor A, Ehnes D, Li Z, Praetorius FM, Gordon M, Yang W, Coventry B, Hicks DR, Cao L, Bethel N, Heine P, Murray AN, Gerben S, Carter L, Miranda M, Negahdari B, Lee S, Trapnell C, Stewart L, Ekiert DC, Schlessinger J, Shendure J, Bhabha G, Ruohola-Baker H, Baker D. Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies. bioRxiv, 10.1101/2023.03.14.532666.
[Preprint]
View
| DOI
| Download Preprint (ext.)
2022 | Published | Journal Article | IST-REx-ID: 14282
D. D. Sahtoe et al., “Reconfigurable asymmetric protein assemblies through implicit negative design,” Science, vol. 375, no. 6578. American Association for the Advancement of Science, 2022.
View
| DOI
| PubMed | Europe PMC
2019 | Published | Journal Article | IST-REx-ID: 14299 |

E. FAS et al., “Custom-size, functional, and durable DNA origami with design-specific scaffolds,” ACS Nano, vol. 13, no. 5. ACS Publications, pp. 5015–5027, 2019.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2018 | Published | Journal Article | IST-REx-ID: 14284 |

B. Bräuning et al., “Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB,” Nature Communications, vol. 9. Springer Nature, 2018.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2018 | Published | Thesis | IST-REx-ID: 14306 |

F. M. Praetorius, “Genetically encoding the spatial arrangement of DNA and proteins in self-assembling nanostructures,” Technische Universität München, 2018.
[Published Version]
View
| Download Published Version (ext.)
2017 | Published | Journal Article | IST-REx-ID: 14286
B. Kick, S. Hensler, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Specific growth rate and multiplicity of infection affect high-cell-density fermentation with bacteriophage M13 for ssDNA production,” Biotechnology and Bioengineering, vol. 114, no. 4. Wiley, pp. 777–784, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14287
F. M. Praetorius and H. Dietz, “Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes,” Science, vol. 355, no. 6331. American Association for the Advancement of Science, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14290
F. M. Praetorius, B. Kick, K. L. Behler, M. N. Honemann, D. Weuster-Botz, and H. Dietz, “Biotechnological mass production of DNA origami,” Nature, vol. 552, no. 7683. Springer Nature, pp. 84–87, 2017.
View
| DOI
| PubMed | Europe PMC
2017 | Published | Journal Article | IST-REx-ID: 14309 |

M. Siavashpouri, C. Wachauf, M. Zakhary, F. M. Praetorius, H. Dietz, and Z. Dogic, “Molecular engineering of chiral colloidal liquid crystals using DNA origami,” Nature Materials, vol. 16, no. 8. Springer Nature, pp. 849–856, 2017.
[Preprint]
View
| DOI
| Download Preprint (ext.)
| PubMed | Europe PMC
| arXiv
2017 | Published | Conference Abstract | IST-REx-ID: 14310
M. Siavashpouri, C. Wachauf, M. Zakhary, F. M. Praetorius, H. Dietz, and Z. Dogic, “Molecular engineering of colloidal liquid crystals using DNA origami,” in APS March Meeting 2017, 2017.
View
2016 | Published | Journal Article | IST-REx-ID: 14302
E. Stahl, F. M. Praetorius, C. C. de Oliveira Mann, K.-P. Hopfner, and H. Dietz, “Impact of heterogeneity and lattice bond strength on DNA triangle crystal growth,” ACS Nano, vol. 10, no. 10. American Chemical Society, pp. 9156–9164, 2016.
View
| DOI
| PubMed | Europe PMC
2016 | Published | Journal Article | IST-REx-ID: 14304
T. G. Martin et al., “Design of a molecular support for cryo-EM structure determination,” PNAS, vol. 113, no. 47. Proceedings of the National Academy of Sciences, pp. E7456–E7463, 2016.
[Published Version]
View
| DOI
| PubMed | Europe PMC
2015 | Published | Journal Article | IST-REx-ID: 14303 |

B. Kick, F. M. Praetorius, H. Dietz, and D. Weuster-Botz, “Efficient production of single-stranded phage DNA as scaffolds for DNA origami,” Nano Letters, vol. 15, no. 7. ACS Publications, pp. 4672–4676, 2015.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2014 | Published | Journal Article | IST-REx-ID: 14301 |

E. Stahl, T. Martin, F. M. Praetorius, and H. Dietz, “Facile and scalable preparation of pure and dense DNA origami solutions,” Angewandte Chemie International Edition, vol. 126, no. 47. Wiley, pp. 12949–12954, 2014.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC
2011 | Published | Journal Article | IST-REx-ID: 14305 |

A. Bachmann, D. Wildemann, F. M. Praetorius, G. Fischer, and T. Kiefhaber, “Mapping backbone and side-chain interactions in the transition state of a coupled protein folding and binding reaction,” PNAS, vol. 108, no. 10. Proceedings of the National Academy of Sciences, pp. 3952–3957, 2011.
[Published Version]
View
| DOI
| Download Published Version (ext.)
| PubMed | Europe PMC