Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

Ruprecht, Verena; Wieser, Stefan; Callan Jones, Andrew; Smutny, Michael; Morita, Hitoshi; Sako, Keisuke; Barone, Vanessa; Ritsch Marte, Monika; Sixt, Michael K; Voituriez, Raphaël; Heisenberg, Carl-Philipp J

Cortical contractility triggers a stochastic switch to fast amoeboid cell motility

Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V, Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.

Download

IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf 4.36 MB [Published Version]

DOI

10.1016/j.cell.2015.01.008

Journal Article | Published | English

Scopus indexed

Author

Ruprecht, Verena^ISTA ; Wieser, Stefan^ISTA ; Callan Jones, Andrew; Smutny, Michael^ISTA ; Morita, Hitoshi^ISTA; Sako, Keisuke^ISTA ; Barone, Vanessa^ISTA ; Ritsch Marte, Monika; Sixt, Michael K^ISTA ; Voituriez, Raphaël; Heisenberg, Carl-Philipp ^ISTA

Corresponding author has ISTA affiliation

Department

Heisenberg Group
Sixt Group

Grant

Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation

Abstract

3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

Publishing Year

2015

Date Published

2015-02-12

Journal Title

Cell

Publisher

Cell Press

Acknowledgement

We would like to thank R. Hausschild and E. Papusheva for technical assistance and the service facilities at the IST Austria for continuous support. The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel and E. Paluch for exchanging unpublished data.

Acknowledged SSUs

Scientific Service Units (SSUs)

Volume

160

Issue

Page

673 - 685

IST-REx-ID

1537

Cite this

Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 2015;160(4):673-685. doi:10.1016/j.cell.2015.01.008

Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K., … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.008

Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.01.008.

V. Ruprecht et al., “Cortical contractility triggers a stochastic switch to fast amoeboid cell motility,” Cell, vol. 160, no. 4. Cell Press, pp. 673–685, 2015.

Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell, vol. 160, no. 4, Cell Press, 2015, pp. 673–85, doi:10.1016/j.cell.2015.01.008.

All files available under the following license(s):

Creative Commons Attribution 4.0 International Public License (CC-BY 4.0):