Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice
Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 5(3), 103157.
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Abstract
The generation of diverse cell types during development is fundamental to brain
functions. We outline a protocol to quantitatively assess the clonal output of individual neural progenitors using mosaic analysis with double markers (MADM) in
mice. We first describe steps to acquire and reconstruct adult MADM clones in
the superior colliculus. Then we detail analysis pipelines to determine clonal
composition and architecture. This protocol enables the buildup of quantitative
frameworks of lineage progression with precise spatial resolution in the brain.
For complete details on the use and execution of this protocol, please refer to
Cheung et al.1
Publishing Year
Date Published
2024-07-04
Journal Title
STAR Protocols
Acknowledgement
We thank A. Heger for mouse breeding support. This work was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging & Optics and Preclinical facilities. G.C. received funding from the European Commission (IST plus postdoctoral fellowship); S.H. was funded by ISTA institutional funds and the Austrian Science Fund Special Research Programmes (FWF SFB-F78 Neuro Stem Modulation).
Acknowledged SSUs
Volume
5
Issue
3
Article Number
103157
eISSN
IST-REx-ID
Cite this
Cheung GT, Streicher C, Hippenmeyer S. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 2024;5(3). doi:10.1016/j.xpro.2024.103157
Cheung, G. T., Streicher, C., & Hippenmeyer, S. (2024). Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. https://doi.org/10.1016/j.xpro.2024.103157
Cheung, Giselle T, Carmen Streicher, and Simon Hippenmeyer. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” STAR Protocols, 2024. https://doi.org/10.1016/j.xpro.2024.103157.
G. T. Cheung, C. Streicher, and S. Hippenmeyer, “Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice,” STAR Protocols, vol. 5, no. 3. 2024.
Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 5(3), 103157.
Cheung, Giselle T., et al. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” STAR Protocols, vol. 5, no. 3, 103157, 2024, doi:10.1016/j.xpro.2024.103157.
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