Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice

Cheung, Giselle T; Streicher, Carmen; Hippenmeyer, Simon

Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice

Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 5(3), 103157.

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https://doi.org/10.1016/j.xpro.2024.103157 [Published Version]

DOI

10.1016/j.xpro.2024.103157

Journal Article | Epub ahead of print | English

Scopus indexed

Author

Cheung, Giselle T^ISTA ; Streicher, Carmen^ISTA; Hippenmeyer, Simon^ISTA

Corresponding author has ISTA affiliation

Department

Hippenmeyer Group

Grant

ISTplus - Postdoctoral Fellowships
Molecular Mechanisms of Neural Stem Cell Lineage Progression

Abstract

The generation of diverse cell types during development is fundamental to brain functions. We outline a protocol to quantitatively assess the clonal output of individual neural progenitors using mosaic analysis with double markers (MADM) in mice. We first describe steps to acquire and reconstruct adult MADM clones in the superior colliculus. Then we detail analysis pipelines to determine clonal composition and architecture. This protocol enables the buildup of quantitative frameworks of lineage progression with precise spatial resolution in the brain. For complete details on the use and execution of this protocol, please refer to Cheung et al.1

Publishing Year

2024

Date Published

2024-07-04

Journal Title

STAR Protocols

Publisher

Elsevier

Acknowledgement

We thank A. Heger for mouse breeding support. This work was supported by the Scientific Service Units of IST Austria through resources provided by the Imaging & Optics and Preclinical facilities. G.C. received funding from the European Commission (IST plus postdoctoral fellowship); S.H. was funded by ISTA institutional funds and the Austrian Science Fund Special Research Programmes (FWF SFB-F78 Neuro Stem Modulation).

Acknowledged SSUs

Imaging & Optics Facility
Pre-Clinical Facility

Volume

Issue

Article Number

103157

eISSN

2666-1667

IST-REx-ID

17187

Cite this

Cheung GT, Streicher C, Hippenmeyer S. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 2024;5(3). doi:10.1016/j.xpro.2024.103157

Cheung, G. T., Streicher, C., & Hippenmeyer, S. (2024). Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. Elsevier. https://doi.org/10.1016/j.xpro.2024.103157

Cheung, Giselle T, Carmen Streicher, and Simon Hippenmeyer. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” STAR Protocols. Elsevier, 2024. https://doi.org/10.1016/j.xpro.2024.103157.

G. T. Cheung, C. Streicher, and S. Hippenmeyer, “Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice,” STAR Protocols, vol. 5, no. 3. Elsevier, 2024.

Cheung GT, Streicher C, Hippenmeyer S. 2024. Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice. STAR Protocols. 5(3), 103157.

Cheung, Giselle T., et al. “Protocol for Quantitative Reconstruction of Cell Lineage Using Mosaic Analysis with Double Markers in Mice.” STAR Protocols, vol. 5, no. 3, 103157, Elsevier, 2024, doi:10.1016/j.xpro.2024.103157.

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