A framework for neural organoids, assembloids and transplantation studies
Pașca SP, Arlotta P, Bateup HS, Camp JG, Cappello S, Gage FH, Knoblich JA, Kriegstein AR, Lancaster MA, Ming GL, Novarino G, Okano H, Parmar M, Park IH, Reiner O, Song H, Studer L, Takahashi J, Temple S, Testa G, Treutlein B, Vaccarino FM, Vanderhaeghen P, Young-Pearse T. 2025. A framework for neural organoids, assembloids and transplantation studies. Nature. 639(8054), 315–320.
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Journal Article
| Published
| English
Scopus indexed
Author
Pașca, Sergiu P.;
Arlotta, Paola;
Bateup, Helen S.;
Camp, J. Gray;
Cappello, Silvia;
Gage, Fred H.;
Knoblich, Jürgen A.;
Kriegstein, Arnold R.;
Lancaster, Madeline A.;
Ming, Guo Li;
Novarino, GaiaISTA 
;
Okano, Hideyuki
All
;
Okano, Hideyuki
All
Department
Abstract
As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.
Publishing Year
Date Published
2025-03-13
Journal Title
Nature
Publisher
Springer Nature
Acknowledgement
The authors thank members of their laboratories who provided feedback on earlier versions of this manuscript, including A. Jourdon, V. Mariano, T. L. Li, N. Caporale, E. Villa and M. Sutcliffe.
Volume
639
Issue
8054
Page
315-320
ISSN
eISSN
IST-REx-ID
Cite this
Pașca SP, Arlotta P, Bateup HS, et al. A framework for neural organoids, assembloids and transplantation studies. Nature. 2025;639(8054):315-320. doi:10.1038/s41586-024-08487-6
Pașca, S. P., Arlotta, P., Bateup, H. S., Camp, J. G., Cappello, S., Gage, F. H., … Young-Pearse, T. (2025). A framework for neural organoids, assembloids and transplantation studies. Nature. Springer Nature. https://doi.org/10.1038/s41586-024-08487-6
Pașca, Sergiu P., Paola Arlotta, Helen S. Bateup, J. Gray Camp, Silvia Cappello, Fred H. Gage, Jürgen A. Knoblich, et al. “A Framework for Neural Organoids, Assembloids and Transplantation Studies.” Nature. Springer Nature, 2025. https://doi.org/10.1038/s41586-024-08487-6.
S. P. Pașca et al., “A framework for neural organoids, assembloids and transplantation studies,” Nature, vol. 639, no. 8054. Springer Nature, pp. 315–320, 2025.
Pașca SP, Arlotta P, Bateup HS, Camp JG, Cappello S, Gage FH, Knoblich JA, Kriegstein AR, Lancaster MA, Ming GL, Novarino G, Okano H, Parmar M, Park IH, Reiner O, Song H, Studer L, Takahashi J, Temple S, Testa G, Treutlein B, Vaccarino FM, Vanderhaeghen P, Young-Pearse T. 2025. A framework for neural organoids, assembloids and transplantation studies. Nature. 639(8054), 315–320.
Pașca, Sergiu P., et al. “A Framework for Neural Organoids, Assembloids and Transplantation Studies.” Nature, vol. 639, no. 8054, Springer Nature, 2025, pp. 315–20, doi:10.1038/s41586-024-08487-6.
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