Structures of multisubunit membrane complexes with the CRYO ARM 200

Gerle, Christoph; Kishikawa, Jun-ichi; Yamaguchi, Tomoko; Nakanishi, Atsuko; Çoruh, Mehmet Orkun; Makino, Fumiaki; Miyata, Tomoko; Kawamoto, Akihiro; Yokoyama, Ken; Namba, Keiichi; Kurisu, Genji; Kato, Takayuki

Structures of multisubunit membrane complexes with the CRYO ARM 200

Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. 2022. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 71(5), 249–261.

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DOI

10.1093/jmicro/dfac037

Journal Article | Published | English

Scopus indexed

Author

Gerle, Christoph; Kishikawa, Jun-ichi; Yamaguchi, Tomoko; Nakanishi, Atsuko; Çoruh, Mehmet Orkun^ISTA ; Makino, Fumiaki; Miyata, Tomoko; Kawamoto, Akihiro; Yokoyama, Ken; Namba, Keiichi; Kurisu, Genji; Kato, Takayuki
All

Department

Sazanov Group

Abstract

Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica.

Keywords

Radiology; Nuclear Medicine and imaging; Instrumentation; Structural Biology

Publishing Year

2022

Date Published

2022-10-01

Journal Title

Microscopy

Acknowledgement

Cyclic Innovation for Clinical Empowerment (JP17pc0101020 from Japan Agency for Medical Research and Development (AMED) to K.N. and G.K.); Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research) from AMED (JP20am0101117 to K.N., JP16K07266 to Atsunori Oshima and C.G., JP22ama121001j0001 to Masaki Yamamoto, G.K., T.K. and C.G.); a JSPS KAHKENHI grant (20K06514 to J.K.) and a Grant-in-aid for JSPS fellows (20J00162 to A.N.). We are grateful for initiation and scientific support from Matthias Rogner, Marc M. Nowaczyk, Anna Frank and ̈Yuko Misumi for the PSI monomer project and also would like to thank Hideki Shigematsu for critical reading of the manuscript. And we are indebted to the two anonymous reviewers who helped us to improve our manuscript.

Volume

Issue

Page

249-261

ISSN

2050-5698

eISSN

2050-5701

IST-REx-ID

11648

Cite this

Gerle C, Kishikawa J, Yamaguchi T, et al. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 2022;71(5):249-261. doi:10.1093/jmicro/dfac037

Gerle, C., Kishikawa, J., Yamaguchi, T., Nakanishi, A., Çoruh, M. O., Makino, F., … Kato, T. (2022). Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. Oxford University Press. https://doi.org/10.1093/jmicro/dfac037

Gerle, Christoph, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Mehmet Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy. Oxford University Press, 2022. https://doi.org/10.1093/jmicro/dfac037.

C. Gerle et al., “Structures of multisubunit membrane complexes with the CRYO ARM 200,” Microscopy, vol. 71, no. 5. Oxford University Press, pp. 249–261, 2022.

Gerle, Christoph, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy, vol. 71, no. 5, Oxford University Press, 2022, pp. 249–61, doi:10.1093/jmicro/dfac037.

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